We have recently published a new protocol in STAR protocols, a journal by Cell Press. This protocol provides an in-depth, step-by-step protocol describing how to perform single-molecule Fluorescence Recovery After Photobleaching (smFRAP). This technique was developed as a method to determine the relative concentrations of nuclear envelope transmembrane proteins (NETs) Read more…
My colleague Yichen Li and I co-wrote an article recently published in Nature Protocols describing how to utilize SPEED microscopy to derive 3D information from 2D data in rotationally symmetric structures. The article is titled: High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation and can Read more…
We recently were invited to co-author a book chapter in the book A Closer Look at Membrane Proteins edited by Simon Patching. Our group wrote the second chapter entitled Imaging Transmembrane Protein Transport Across the Nuclear Envelope. The book is free and available online. Read The Book Download A Closer Read more…
We have just published a new article on how to differentiate between inner and outer nuclear membrane transmembrane proteins. These techniques include immunogold labeled electron microscopy, super resolution microscopy techniques, single-molecule microscopy and more. It has been a pleasure to work with my co-authors and I hope that you enjoy Read more…
Our lab has recently published an article on a 3D tracking free approach to obtaining 3D super-resolution information in rotaionally symmetric bio-structures. This approach provides a method of extrapolating 3D information from 2D super-resolution information within a rotaionally symmetric bio-structure. By rotationally symmetric, what is meant is a channel which Read more…