My colleague Yichen Li and I co-wrote an article recently published in Nature Protocols describing how to utilize SPEED microscopy to derive 3D information from 2D data in rotationally symmetric structures. The article is titled: High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation and can be downloaded here free of charge.
We have recently published a new protocol in STAR protocols, a journal by Cell Press. This protocol provides an in-depth, step-by-step protocol describing how to perform single-molecule Fluorescence Recovery After Photobleaching (smFRAP). This technique was Read more…
We recently were invited to co-author a book chapter in the book A Closer Look at Membrane Proteins edited by Simon Patching. Our group wrote the second chapter entitled Imaging Transmembrane Protein Transport Across the Read more…
We have just published a new article on how to differentiate between inner and outer nuclear membrane transmembrane proteins. These techniques include immunogold labeled electron microscopy, super resolution microscopy techniques, single-molecule microscopy and more. It Read more…